Azocarmine
Azocarmine is a synthetic dye that belongs to the group of azo dyes. It has the chemical formula C26H15N4NaO9S2 and is also known as C.I. Acid Red 103. It is a deep red dye that is soluble in water and can be used in various aqueous or alcoholic solutions.
In histology, azo carmine is often used as a basic dye that is used to stain acidic cellular components, such as nucleic acids or acidic proteins. It has a strong affinity for these structures and gives them an intense red color. A common use of azocarmine is in azane trichrome staining, where it is used with other stains such as aniline blue and orange G to selectively stain various cell structures and tissue types.
In a 0.1% solution with some acetic acid added, azocarmine provides a dilute yet effective staining solution for histological applications. The addition of acetic acid causes a slight acidification of the solution, which affects the staining properties of azocarmine and increases its binding affinity to acidic cellular components.
Using a 0.1% azocarmine solution allows for more precise and selective staining of tissue structures, as the lower concentration of dye reduces the risk of overstaining. Combined with the acidic environment provided by the acetic acid, this results in better delineation and contrast of different cell structures and tissue types, allowing for more accurate assessment and diagnosis.
The addition of acetic acid also helps to remove dye residues that have accumulated during the staining process and improves the durability of the stain.
Azocarmine can also be used in combination with other dyes in multicolor staining solutions to provide differential staining of different cell structures and tissue types.
In histology, azo carmine is often used as a basic dye that is used to stain acidic cellular components, such as nucleic acids or acidic proteins. It has a strong affinity for these structures and gives them an intense red color. A common use of azocarmine is in azane trichrome staining, where it is used with other stains such as aniline blue and orange G to selectively stain various cell structures and tissue types.
In a 0.1% solution with some acetic acid added, azocarmine provides a dilute yet effective staining solution for histological applications. The addition of acetic acid causes a slight acidification of the solution, which affects the staining properties of azocarmine and increases its binding affinity to acidic cellular components.
Using a 0.1% azocarmine solution allows for more precise and selective staining of tissue structures, as the lower concentration of dye reduces the risk of overstaining. Combined with the acidic environment provided by the acetic acid, this results in better delineation and contrast of different cell structures and tissue types, allowing for more accurate assessment and diagnosis.
The addition of acetic acid also helps to remove dye residues that have accumulated during the staining process and improves the durability of the stain.
Azocarmine can also be used in combination with other dyes in multicolor staining solutions to provide differential staining of different cell structures and tissue types.
Article no.: 10147
Staining of tissue samples
product information
Relevant Ingredients:
• Azocarmine G (C.I.: 50085)
Hazard and safety instructions
Suggested products
-
Staining Kit: AZAN after HEIDENHAIN
Staining of tissue samples
Article number: 12079from € 67.00 + VATStaining Kit: MOLLIER’s Fourfold-Staining
Dyeing elastic fibers
Article number: 12477from € 176.20 + VATPinned products0
Last seen1