Chromotrope Aniline Blue Solution
Chromotrope aniline blue solution is used for histological examination of polysaccharides such as chitin, cellulose and lignin. This staining method is particularly important in the study of plant structures and fungi, where such polysaccharides are relevant.
In the chemical functioning of this solution, the combination of chromotrope 2R and aniline blue w.s. play a crucial role. By Chromotrope 2R, mainly acidic polysaccharides are stained, while Aniline Blue w.s. has an affinity for basic polysaccharides and glycoproteins. The hydrochloric acid aids staining and increases the selectivity of dye binding to target structures.
The special suitability lies in the ability to visualize detailed differences in polysaccharide structures of various plant and fungal tissues. The aqueous base allows easy handling and uniform distribution of the solution in the tissue. This can provide important information on the structure and composition of biological samples, which is important for the study of developmental and metabolic processes as well as possible pathologies.
In the chemical functioning of this solution, the combination of chromotrope 2R and aniline blue w.s. play a crucial role. By Chromotrope 2R, mainly acidic polysaccharides are stained, while Aniline Blue w.s. has an affinity for basic polysaccharides and glycoproteins. The hydrochloric acid aids staining and increases the selectivity of dye binding to target structures.
The special suitability lies in the ability to visualize detailed differences in polysaccharide structures of various plant and fungal tissues. The aqueous base allows easy handling and uniform distribution of the solution in the tissue. This can provide important information on the structure and composition of biological samples, which is important for the study of developmental and metabolic processes as well as possible pathologies.
Article no.: 13053
Staining of tissue samples
product information
Relevant Ingredients:
• Chromotrope 2R (C.I.: 16570)
• Aniline blue w.s. (C.I.: 42755 / 42780)
• Hydrochloric Acid 37%
Hazard and safety instructions
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Staining Kit: ROQUE’s Trichrome Staining (CAB)
Staining of tissue samples
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