Staining Kit: Van GIESON’s after HANSEN
The Van Gieson staining kit according to Hansen is a histological staining kit used to distinguish collagen fibers and muscle tissue in histological sections. The staining is based on the combination of Weigert's iron hematoxylin and Hansen's picrofuchsin.
The essential components of this staining kit are:
1. Weigert stock solution A (Item No.: 10225A) and Weigert stock solution B (Item No.: 10225B): These stock solutions are combined to prepare Weigert's ferric hematoxylin solution, which is used to stain cell nuclei. The nuclei will appear black after staining.
2. Hydrochloric Acid Alcohol 1% (Item No.: 10372): This solution is used to differentiate the stain to remove excess hematoxylin and ensure a clear background.
3. Hansen's Picrofuchsin (Item No.: 10345): A mixture of picric acid and acid fuchsin used to stain collagen fibers and muscle tissue. Collagen fibers appear red, while muscle tissue is visualized in a reddish-purple color.
Hansen's Van Gieson stain is a useful technique for visualizing collagen and muscle tissue in histologic sections and provides a simple and rapid method for clearly distinguishing different tissue components. The stain can be used in histopathology, research and diagnostics.
Article no.: 11099
Staining of tissue samples
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product information
Components of this kit:
• WEIGERT stock solution A, Artikel-Nr.:10225A
• WEIGERT stock solution B, Artikel-Nr.:10225B
• Hydrochloric acid Alcohol (1 % / 70 %), Artikel-Nr.:10372
• HANSEN’s Picric Fuchsine, Artikel-Nr.:10345
Instructions / Protocol / Recommendations
Verwendung:
The Van Gieson staining kit according to Hansen is a histological staining kit for in vitro diagnostics. It is used to differentiate between collagen fibers and muscle tissue in histological sections. By combining Weigert's iron hematoxylin and Hansen's picrofuchsin, cell nuclei are stained black, collagen fibers red, and muscle tissue red-violet. Differentiation with hydrochloric acid alcohol 1% removes excess hematoxylin and ensures a clear background. This staining is used in histopathology, research and diagnostics for visualization of tissue components.
Prinzip:
The Van Gieson staining kit according to Hansen is a histological staining protocol used in in vitro diagnostics to visualize tissue or cell structures. Weigert stock solutions A and B provide staining of cell nuclei, while hydrochloric acid alcohol contributes to decolorization and differentiation. Picrofuchsin according to Hansen selectively stains collagen fibers and elastin. The different staining components provide contrast and differentiation of specific tissue and cell structures, enabling precise analysis and diagnostics.
Verfahren:
The Van Gieson staining kit according to Hansen is a histological staining protocol used in in vitro diagnostics to visualize tissue or cell structures. Weigert stock solutions A and B provide staining of cell nuclei, while hydrochloric acid alcohol contributes to decolorization and differentiation. Picrofuchsin according to Hansen selectively stains collagen fibers and elastin. The different staining components provide contrast and differentiation of specific tissue and cell structures, enabling precise analysis and diagnostics.
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Signal Word 2: flamme, exclam, acid_red, |
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