Formaline-free Zinc Fixative (for IHC)
Formalin-free Zinc Fixative (for IHC) serves as a fixative solution in immunohistochemistry, where the absence of formalin reduces potential health risks and the need for special handling protocols.
The particular suitability of this fixative lies in its ability to preserve tissue quality and antigenicity, making it an attractive option for applications requiring high morphological and molecular preservation. Due to its composition, particularly the zinc chloride and zinc acetate, it creates a fixative solution that provides excellent tissue preservation.
The chemical function of this fixative is based on the interaction of zinc ions with protein structures in the tissue, resulting in fixation of the tissue structure. The zinc salts it contains, zinc acetate and zinc chloride, act synergistically, while calcium acetate and TRIS optimize the pH.
The operation of this formalin-free zinc fixative is similar to that of formaldehyde fixatives, with the advantage that it is less toxic and has fewer negative side effects. Fixation without formalin better preserves antigens in cells, which can lead to higher specificity and sensitivity in subsequent antibody detection. Overall, this fixative is ideally suited for use in a variety of diagnostic and scientific applications where antigen preservation and high fixation quality are essential.
The particular suitability of this fixative lies in its ability to preserve tissue quality and antigenicity, making it an attractive option for applications requiring high morphological and molecular preservation. Due to its composition, particularly the zinc chloride and zinc acetate, it creates a fixative solution that provides excellent tissue preservation.
The chemical function of this fixative is based on the interaction of zinc ions with protein structures in the tissue, resulting in fixation of the tissue structure. The zinc salts it contains, zinc acetate and zinc chloride, act synergistically, while calcium acetate and TRIS optimize the pH.
The operation of this formalin-free zinc fixative is similar to that of formaldehyde fixatives, with the advantage that it is less toxic and has fewer negative side effects. Fixation without formalin better preserves antigens in cells, which can lead to higher specificity and sensitivity in subsequent antibody detection. Overall, this fixative is ideally suited for use in a variety of diagnostic and scientific applications where antigen preservation and high fixation quality are essential.
Article no.: 14064
Fixation of tissue samples
product information
Relevant Ingredients:
• Calcium acetate
• acetic acid ; zinc (II)-compound
• Zinc chloride
• TRIS
• Hydrochloric Acid 1.0 mol/l
Instructions / Protocol / Recommendations
Verwendung:
Bei der Fixierung werden alle in der Zelle ablaufenden Prozesse unterbrochen, unter möglichst optimaler Erhaltung des Zustandes und der Struktur des Gewebes. Biochemische Prozesse wie Autolyse, Verwesung und Fäulnis werden wirkungsvoll verhindert. Fixierlösung nach Stieve eignet sich zur Fixierung von Hodengewebe und Eierstockgewebe. Die Fixierlösung ist ausschließlich für die professionelle Anwendung als in-vitro Diagnostikum im histologischen Labor vorgesehen.