Cresyl Fast Violet for KLUEVER BARRERA
Kresylecht violet is a dye used in Klüver-Barrera staining, a histological staining technique for visualizing neurons and nerve fibers in nervous tissue. In this staining method, Kresylecht violet is used together with Luxol Fast Blue (LFB). Luxol Fast Blue stains myelin, the lipid sheath around nerve fibers, blue, while Kresylechtviolet stains Nissl substance in neurons and glial cells purple.
The Kresylechtviolet solution is used in Klüver-Barrera staining after the application of Luxol Fast Blue. The combination of these two stains allows both myelin sheaths and cell bodies of neurons and glial cells to be clearly distinguished. This technique is particularly useful in the study of central and peripheral nervous system diseases, as it provides detailed information about the morphology and distribution of neurons and nerve fibers.
To perform Klüver-Barrera staining, tissue sections are first stained in a Luxol Fast Blue solution, followed by treatment with lithium carbonate or ethanol to differentiate and remove excess LFB. The sections are then stained in a Kresylecht violet solution, which stains the Nissl substance violet. After staining is complete, the sections are dehydrated in ascending concentrations of alcohol, clarified in xylene, and finally mounted in appropriate mounting medium to prepare them for microscopic examination.
The Kresylechtviolet solution is used in Klüver-Barrera staining after the application of Luxol Fast Blue. The combination of these two stains allows both myelin sheaths and cell bodies of neurons and glial cells to be clearly distinguished. This technique is particularly useful in the study of central and peripheral nervous system diseases, as it provides detailed information about the morphology and distribution of neurons and nerve fibers.
To perform Klüver-Barrera staining, tissue sections are first stained in a Luxol Fast Blue solution, followed by treatment with lithium carbonate or ethanol to differentiate and remove excess LFB. The sections are then stained in a Kresylecht violet solution, which stains the Nissl substance violet. After staining is complete, the sections are dehydrated in ascending concentrations of alcohol, clarified in xylene, and finally mounted in appropriate mounting medium to prepare them for microscopic examination.
Article no.: 11207
Staining of tissue samples
product information
Relevant Ingredients:
• Cresyl Fast Violet