Hydrochloric Acid in Ethanol (0.4 % / 70 %)

The hydrochloric acid-alcohol solution (0.4% / 70%) is a widely used solution in histology, medical diagnostics and life sciences. Its application enables the improvement of staining results as well as the removal of unwanted staining from histological specimens.

The solution is particularly suitable for the differentiation of cell structures in tissue samples. Through targeted interaction with specific structures, the contrast in microscopic analyses can be optimized. With longer exposure times, the solution can also be used to decolorize samples, correcting undesirable staining.

The chemical properties of the hydrochloric acid-alcohol solution result from the combination of ethanol, hydrochloric acid, and water. This mixture exhibits a slightly acidic nature, which is largely responsible for the differentiating effect. During use, the acidic components of the solution interact with the basic structures of the tissue specimens, allowing selective staining and destaining.

In terms of how it works, the hydrochloric acid-alcohol solution provides an effective way to selectively interact cell structures within histological specimens. The chemical reactions that occur during the staining and destaining process aid in the identification and separation of cells and tissue components, contributing to improved visualization and analysis.