PBS Buffer - 10x Concentrate
The 10-fold concentrated PBS buffer concentrate is a basic reagent in biological laboratories, known for its wide range of applications. It is often used as a rinsing solution and to maintain pH in cell cultures, as well as a buffer solution for biochemical reactions. Its particular suitability lies in its ability to keep the environment of cells and tissues stable during experiments, allowing reliable and reproducible results to be obtained.
Chemically, PBS buffer concentrate consists of the salts sodium chloride, di-sodium hydrogen phosphate dihydrate, potassium chloride and potassium dihydrogen phosphate dissolved in water. The ratio of these salts has been carefully selected to achieve a pH of 7.4 at room temperature, which is the pH of most living cells.
The mode of action of the PBS buffer is based on its ability to regulate the proton balance in solution. This allows the pH to remain stable even when acids or bases are added. In the medical field and in the life sciences, PBS buffer concentrate is used to prepare samples for microscopy, in cell culture and for immunohistochemical procedures, as it preserves the structure and function of cells during experiments.
Chemically, PBS buffer concentrate consists of the salts sodium chloride, di-sodium hydrogen phosphate dihydrate, potassium chloride and potassium dihydrogen phosphate dissolved in water. The ratio of these salts has been carefully selected to achieve a pH of 7.4 at room temperature, which is the pH of most living cells.
The mode of action of the PBS buffer is based on its ability to regulate the proton balance in solution. This allows the pH to remain stable even when acids or bases are added. In the medical field and in the life sciences, PBS buffer concentrate is used to prepare samples for microscopy, in cell culture and for immunohistochemical procedures, as it preserves the structure and function of cells during experiments.
Article no.: 13684
Preparation of buffer solutions
product information
Relevant Ingredients:
• Sodium chloride
• Di-sodium hydrogen phosphate dihydrate
• Potassium chloride
• Potassium dihydrogen phosphate