PBS Buffer pH 7.45
PBS buffer pH 7.45 is widely used in biological research. Its main task is to keep the pH value stable in an environment, which is especially crucial in biological and biochemical experiments.
The chemical and physical properties of the PBS buffer enable this stability. The phosphate salts it contains act as an acid-base pair and can absorb or release excess protons to compensate for fluctuations in pH. Sodium chloride provides appropriate ionic strength in this process, which affects the osmotic conditions in the experiment.
The PBS buffer is widely used for reconstitution of cells, dilution of samples, and washing of cells in in vitro experiments. It provides the ability to treat biological samples under conditions similar to those found in living organisms, contributing to reliable and reproducible results.
The molarity of this PBS buffer is 0.01 M, the osmolarity is 290 mOsm/l, and the buffer capacity ranges from pH 7.2 to 7.8.
The chemical and physical properties of the PBS buffer enable this stability. The phosphate salts it contains act as an acid-base pair and can absorb or release excess protons to compensate for fluctuations in pH. Sodium chloride provides appropriate ionic strength in this process, which affects the osmotic conditions in the experiment.
The PBS buffer is widely used for reconstitution of cells, dilution of samples, and washing of cells in in vitro experiments. It provides the ability to treat biological samples under conditions similar to those found in living organisms, contributing to reliable and reproducible results.
The molarity of this PBS buffer is 0.01 M, the osmolarity is 290 mOsm/l, and the buffer capacity ranges from pH 7.2 to 7.8.
Article no.: 15384
Use as laboratory reagent
product information
Relevant Ingredients:
• Di-sodium hydrogen phosphate dihydrate
• Sodium dihydrogen phosphate monohydrate
• Sodium chloride