Staining Kit: Van GIESON’s Elastica (EvG)
Resorcin-Fuchsin is a staining solution used for specific staining of elastic fibers in tissue sections. It imparts an intense blue-black color to elastic fibers, making them easy to distinguish from other structures.
Weigert stock solution A and B are mixed together to obtain Weigert iron hematoxylin solution. It is used to stain cell nuclei, giving them a characteristic blue-violet color.
Van Gieson Picrofuchsin Solution is used to stain collagen fibers, which turn a bright red color in tissue sections. It allows collagen fibers to be clearly distinguished from other tissue structures.
The 1% hydrochloric acid alcohol solution is used as an intermediate rinse and to decolorize excess fuchsin to ensure optimal staining and improved contrast of stained structures.
Overall, the Elastica by van Gieson (EvG) staining kit provides accurate and high-contrast visualization of elastic fibers, collagen fibers, and cell nuclei, which is critical for detailed examination and diagnosis of tissue specimens in histology and pathology.
Article no.: 12739
Dyeing elastic fibers
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product information
Components of this kit:
• Resorcin-Fuchsin, alcoholic acc. to WEIGERT, Artikel-Nr.:10354
• WEIGERT stock solution A, Artikel-Nr.:10225A
• WEIGERT stock solution B, Artikel-Nr.:10225B
• Picrofuchsin according to VAN GIESON, Artikel-Nr.:11486
• Hydrochloric acid Alcohol (1 % / 70 %), Artikel-Nr.:10372
Instructions / Protocol / Recommendations
Verwendung:
The Elastica according to van Gieson (EvG) staining kit is a high-quality staining kit for in vitro diagnostics in histology and pathology. It allows precise and high-contrast visualization of elastic fibers, collagen fibers and cell nuclei in histological specimens. The kit contains resorcinol-fuchsin for staining elastic fibers, Weigert stock solutions A and B for staining cell nuclei, Van GIESON microfuchsin solution for staining collagen fibers and 1 % hydrochloric acid alcohol for optimizing the staining result.
Prinzip:
The Elastica-van-Gieson (EvG) staining kit is a combination of different solutions used for histological visualization of elastic fibers and collagen in tissue samples. The resorcinol-fuchsin solution binds to elastic fibers and stains them dark purple, while the Weigert stock solutions A and B serve as oxidizing agents for staining differentiation. Van Gieson microfuchsin solution stains collagen fibers red, and hydrochloric acid alcohol solution is used to decalcify and clarify the tissue sample. In in vitro diagnostics, the EvG staining kit allows detailed study of the structure and organization of elastic and collagen fibers in various tissues.
Verfahren:
The Elastica-van-Gieson (EvG) staining kit is a combination of different solutions used for histological visualization of elastic fibers and collagen in tissue samples. The resorcinol-fuchsin solution binds to elastic fibers and stains them dark purple, while the Weigert stock solutions A and B serve as oxidizing agents for staining differentiation. Van Gieson microfuchsin solution stains collagen fibers red, and hydrochloric acid alcohol solution is used to decalcify and clarify the tissue sample. In in vitro diagnostics, the EvG staining kit allows detailed study of the structure and organization of elastic and collagen fibers in various tissues.
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