EDTA decalcification solution ~ 13 %

EDTA decalcification solution with approximately 13% concentration is used in histology and pathology to prepare bone and calcified tissue specimens for microscopic examination. EDTA (ethylenediaminetetraacetic acid) is a chelating agent that selectively removes calcium ions from tissue, thereby dissolving calcifications.

The 13% EDTA decalcifying solution is prepared by dissolving EDTA in water and then adjusting it to a pH of approximately 7.4. The solution allows gentle decalcification of tissue specimens by extracting calcium ions from the tissue without affecting the morphological and cellular structures.

Because the concentration of the EDTA solution is higher than that of the 10% solution, decalcification can be performed in a shorter time. However, this depends on the size and density of the sample and may still take several days to weeks. The solution should be changed regularly to ensure efficient decalcification.

The 13% EDTA decalcification solution is specifically designed for professional use in histology and pathology laboratories and provides an effective and gentle method for preparing bone and calcified tissue specimens for microscopic examination. The solution is also suitable for decalcification of samples to be prepared for immunohistochemistry or in situ hybridization, as it largely preserves antigenicity and nucleic acids.